Composite

Part:BBa_K3505026:Design

Designed by: Asteria Tsapadikou ,Emmanouil Stylianakis   Group: iGEM20_Thessaly   (2020-10-20)


ParaBAD:RBS- β-arrestin2:TEVp:terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2854
    Illegal PstI site found at 1546
    Illegal PstI site found at 1633
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2854
    Illegal PstI site found at 1546
    Illegal PstI site found at 1633
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1446
    Illegal BamHI site found at 1148
    Illegal XhoI site found at 1843
    Illegal XhoI site found at 2237
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2854
    Illegal PstI site found at 1546
    Illegal PstI site found at 1633
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2854
    Illegal PstI site found at 1546
    Illegal PstI site found at 1633
    Illegal AgeI site found at 983
    Illegal AgeI site found at 1829
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 965
    Illegal SapI.rc site found at 2788
    Illegal SapI.rc site found at 3136


Design Notes

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Source

source

References